Nature Biotechnology 31: 46–53. 10. de Hoon MJL, Imoto S, Nolan J, Miyano S (2004) Open source clustering software. Download: Figure 3. Expression of key patterning genes across early development.Expression levels in the 25 µm timeseries are normalized to the highest expression level at any time pioint. Rather, activity seems to be dependent on the rate of reduction of these complexes, although data suggest an activation even for the platinum(II) species 4 in one of the three spheroid models. However, major limitations of platinum therapy, such as resistance development and dose-limiting side effects, remain.1–4 Some attempts to overcome the disadvantages of cisplatin treatment were made by exploring platinum(IV) compounds. Laser ablation-ICP-MS measurements were performed using 20 μm thin cryosections. Fig. 1 Chemical structures of the platinum(IV) complexes 1–3 under investigation, including 4 as the main platinum(II) metabolite and the reference compound satraplatin.
Drug Delivery Rev., 2012, 64(11), 993–1004 CrossRef CAS PubMed. H. R. Mellor, S. Snelling, M. D. Hall, S. Modok, M. Jaffar, T. W. Hambley and R. Callaghan, Biochem. For the local tumor growth experiments, HT1080 cells (1 × 106) were injected subcutaneously into the right flank. This complex elicited a significant inhibition of tumor growth in vivo superior to 1 and satraplatin. To the best of our knowledge, we are the first to show the activation of platinum(IV) complexes in a 3D CH1/PA-1 in vitro model compared to the corresponding 2D model. These results suggest that activity under hypoxia is not highly dependent on drug accumulation and lipophilicity, but seems to be dependent on the rate of reduction of the applied platinum(IV) compounds.
The camera shares a few features with the high-end Canon EOS 5D Mark II, including movie mode, Live preview, and DiGIC 4. Like the EOS 450D and EOS 1000D, it uses SDHC media storage, and is the third EOS model to use that medium instead of CompactFlash. Genitourin. Cancer, 2011, 9(1), 27–30 CrossRef PubMed. E. Wexselblatt and D. Gibson, J. Inorg. The order of activation in hypoxic spheroids if compared with monolayer culture, however, shows no correlation with accumulation data. Furthermore, we clearly showed that drug penetration is not a reason for differences in the cytotoxicity of platinum(IV) complexes in the HT1080 spheroid model. Methods involving the tagging, sorting, and analysis of RNA from cells in specific spatial domains have shown promise , but remain non-trivial to apply systematically, especially across genotypes and species. Supporting InformationFigure S3. Genes called as patterned by Cuffdiff lacking subset tag in BDGP database.